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List of
publications
peer reviewed
(1998-2010):
2010:
Dreher, C., Hielscher,
R., Prodöhl, A., Hellwig, P., and Schneider, D. ‘Characterization of two
cytochrome b6 proteins from the cyanobacterium Gloeobacter violaceus PCC
7421’ (2010) J.
Bioenergetics and Biomembranes. In Press
Friedrich, T., and
Hellwig, P. ‘Redox-induced conformational changes within the Escherichia
coli NADH ubiquinone oxidoreductase (complex I): An analysis by mutagenesis
and FT-IR spectroscopy’ (2010) BBA Bioenergetics.
Review in press.
Trivella, A., Gaillard,
T., Stote, R. H., and Hellwig, P. 'Far infrared spectra of solid state
aliphatic amino acids in different protonation states' (2010) J. Chem. Phys. In Press
Trivella, A., El
Khoury, Y., Gaillard, T., Y., Stote, R. H., Merino, N., Blanco, F. J. and
Hellwig,'Temperature Dependence of
the Far Infrared Signature of Internal Hydrogen Bonds in Proteins as Probed
for Integrins' (2010) A.I.P.
Conf. Proc. In Press
Zanier, K., Ruhlmann,
C., Melin, F., Masson, M., Ould M’hamed ould Sidi A., Bernard, X., Fischer,
B., Brino, L., Ristriani, T., Rybin, V., Baltzinger, M., Vande Pol, S.,
Hellwig, P., Schultz, P., and Travé, G. 'E6 proteins from diverse
Papillomaviruses self associate both in vitro and in vivo' J. Mol. Biol. (2010) 396(1), 90-104.
Hielscher, R. and
Hellwig, P. 'The temperature dependent hydrogen bonding signature of lipids
monitored in the far infrared domain' (2010) Chemphyschem 11(2), 435-41.
2009:
Voicescu, M., El
Khoury, Y., Martel, D., Heinrich, M. and Hellwig, P. 'Spectroscopic
Analysis of Tyrosine Derivatives: On the Role of the Tyrosine - Histidine
Covalent Linkage in Cytochrome c Oxidase' (2009) J Phys Chem B 113(40),
13429-36.
Wenz, T., Hielscher,
R., Hellwig, P., Schägger, H., Richers, S., Hunte, C. 'Role of
phospholipids in respiratory cytochrome bc1 complex
catalysis and supercomplex formation' (2009) Biochim. Biophys. Acta
Bioenergetics 1787(6), 609-616.
Hielscher, R., Wenz, T.
Hunte, C. and Hellwig, P. 'Monitoring the redox and protonation dependent
contributions of cardiolipin in electrochemically induced FTIR difference
spectra of the cytochrome bc1 complex from yeast' (2009) Biochim.
Biophys. Acta Bioenergetics 1787(6), 617-625.
Voicescu, M., Heinrich,
M., and Hellwig, P. 'Steady-State and Time Resolved Fluorescence Analysis
on Tyrosine-Histidine Model Compounds' (2009) J. of Fluorescence 19(2):257-66.
El Khoury, Y., and
Hellwig, P. 'Infrared Spectroscopic Characterization of
Copper-Polyhistidine from 1800-50 cm-1: Model Systems for the Cu
Coordination' (2009) Biol. Inorg. Chem. 14(1):23-34
2008:
Kohlstädt, M., Dörner,
K., Labatzke, R., Koc, C., Hielscher, R., Schiltz, E., Einsle, O., Hellwig,
P., Friedrich, T., 'Heterologous Production, Isolation, Characterization
and Crystallization of a Soluble Fragment of the NADH:Ubiquinone
Oxidoreductase (Complex I) from Aquifex aeolicus' (2008) Biochemistry
47(49), 13036-13045.
Janzon, J., Yuan, Q.,
Malatesta, F., Hellwig, P., Ludwig, B., Durham, B., Millett, F., 'Probing
the Paracoccus denitrificans cytochrome c1 / c552
interaction by mutagenesis and fast kinetics' (2008) Biochemistry 47(49),
12974-12984.
Dürr, K., Koepke, J.,
Hellwig, P., Müller, H., Angerer, H., Peng, G., Olkhova, E., Richter, OMH,
Ludwig, B., Michel, H., 'A D-pathway mutation decouples the Paracoccus
denitrificans cytochrome c oxidase by altering the side chain
orientation of a distant, conserved glutamate' (2008) J. Mol. Biol. 384(4),
865-77.
Friedrich C.G.,
Quentmeier, A., Bardishewsky, F., Rother, D., Orawski, G., Hellwig, P and
Fischer, J. 'Redox control of chemotrophic sulphur oxidation of Paracoccus
pantotrophus' in Microbial Sulfur Metabolism Editors C. Dahl and C.G.
Friedrich, Springer Verlag 2008, Chapter 12, page 139- 150.
Kleinschroth, T.,
Anderka, Ritter, M., Stocker, A., Link, TA., Ludwig, B., and Hellwig, P.
'Characterization of mutations in crucial residues around the Qo binding
site of the cytochrome BC1 complex from Paracoccus denitrificans'
(2008) FEBS J. 275, 4773-4785.
Pohl, T., Schneider,
D., Hielscher, R., Stolpe, S., Dörner, K., Kohlstädt, M., Böttcher, B.,
Hellwig, P., and Friedrich, T. 'Nucleotide-induced conformational changes
in the Escherichia coli NADH:ubiquinone oxidoreductase (complex I)'
(2008) Biochem. Soc. Transaction 36, 971-5.
Dreher, C., Prodöhl,
A., Hielscher, R., Hellwig, P., Schneider, D. 'Multiple Step Assembly Of
the transmembrane Cytochrome b(6)' (2008) J. Mol. Biol. 382(4),
1057-65.
Dörr, S., Schade, U.,
Hellwig, P. 'Far infrared spectroscopy on hemoproteins: A model compound
study from 1800-100 cm(-1)' (2008) Vibrational Spectroscopy 47(1),
59-65.
Hellwig, P., Böhm, A.,
Pfitzner, U. Mäntele, W. , Ludwig, B. 'Spectroscopic study on the
communication between a heme a3 propionate, Asp399 and
the binuclear center of cytochrome c oxidase from Paracoccus
denitrificans' (2008) Biochim. Biophys. Acta Bioenergetics 1777(2),
220-6.
2007:
Dörr, S., Schade, U.,
Hellwig, P., Ortolani, M., 'Characterisation of the temperature dependent
Fe-imidazol vibrational modes in far infrared' (2007) J. Phys. Chem.111(51),
14418-22.
Prodohl, A., Dreher,
C., Hielscher, R., Hellwig, P. and Schneider, D. 'Heterologous expression
and in vitro assembly of the transmembrane cytochrome b6'
(2007) Protein Expr. Purif. 56(2), 279-285.
Quentmeier, A.,
Janning, P., Hellwig, P. and Friedrich C.G., 'Activation of the
heterodimeric central complex SoxYZ of chemotrophic sulfur oxidation is
linked to a conformational change and SoxY-Y interprotein disulfide
formation' (2007) Biochemistry 46(38), 10990-10998.
Forzi, L., Hellwig, P.,
Thauer, R.K., Sawers, R.G., 'The CO and CN- ligands to the
active site Fe in [NiFe]-hydrogenase of Escherichia coli have
different metabolic origins' (2007) FEBS Lett. 581(17),
3317-3321.
Reijerse, E.J.,
Sommerhalter, M., Hellwig, P., Quentmeier, A., Rother, D., Laurich, C.,
Bothe, E., Lubitz, W., Friedrich, C.G., 'The Unusal Redox Centers of SoxXA,
a Novel c-Type Heme-Enzyme Essential for Chemotrophic Sulfur-Oxidation of Paracoccus
pantotrophus' (2007) Biochemistry 46(26), 7804-7810.
Yang, K., Zhang, J;,
Vakkosoglu, A., Hielscher, R., Osborne, JR., Hemp, J., Miyoshi, H.,
Hellwig, P., and Gennis, R.B., ‘Glutamate 107 in subunit I of the
cytochrome bd quinol oxidase from Escherichia coli is
protonated and is near the heme d/heme b595
binuclear center’ (2007) Biochemistry 46(11), 3270-3278.
Wenz, T., Covian, R.,
Hellwig, P., MacMillan, F., Meunier, B., Trumpower, B.L. and Hunte, C.,
‘Mutational analysis of cytrochrome b at the ubiquinol oxidation
site of yeast complex III' (2007) J. Biol. Chem. 282(6),
3977-3988.
2006:
Li, WW., Hellwig, P.,
Ritter, M. and Hähnel, W. ‘De novo design, synthesis and characterization
of quinoproteins’ (2006)Chemistry, A European Journal 12(27),
7236-7245.
Maklashina, E.,
Hellwig, P., Rothery, R.A.,Kotlyar, V., Sher, Y., Weiner, J.H., Cecchini,
G., ’Differences in protonationof ubiquinone and menaquinone in fumarate
reductase from Escherichia coli’(2006)J. Biol. Chem. 281,
26655-26664.
Flemming, D., Hellwig,
P., Lepper, S., Kloer,D.P., Friedrich, T., ‘Catalytic importance of acidic
amino acids on subunitNuoB of the Escherichia coli NADH: Ubiquinone
oxidoreductase (complex I)’(2006) J. Biol. Chem. 281,
24781-24789.
Wenz, T., Hellwig, P.,
MacMillan, F., Meunier,B., Hunte, C., ‘Probing the role of E272 in quinol
oxidation of mitochondrialcomplex III’ (2006) Biochemistry 45,
9042-9052.
Wolpert, M., Hellwig,
P.,‘Infrared spectra and molar absorptioncoefficients of the 20 alpha amino
acids in aqueous solutions in the spectralrange from 1800 to 500 cm-1’
(2006) Spectrochimica Acta A: Mol. & Biomol. 64,
987-1001.
Dörr, S., Wolpert, M.,
Hellwig, P., ‘Study onthe redox state dependent γ(CH) vibrational modes of the
c-type heme’(2006) Biopolymers 82, 349-352.
Hielscher, R., Wenz,
T., Stolpe, S., Hunte, C.,Friedrich, T., Hellwig, P., ‘Monitoring
redox-dependent contribution of lipidsin Fourier transform infrared
difference spectra of complex I from Escherichiacoli’ (2006) Biopolymers
82, 291-294.
2005:
Flemming, D., Stolpe,
S., Schneider, D.,Hellwig, P., Friedrich, T., ‘A possible role for
iron-sulfur cluster N2 inproton translocation by the NADH: Ubiquinone
oxidoreductase (Complex I)’ (2005)J. Mol. Microbiol. Biotechn. 10,
208-222.
Friedrich, T., Stolpe,
S., Schneider, D.,Barquera, B., Hellwig, P., ‘Ion translocation by the
Escherichia coliNADH:ubiquinone oxidoreductase (complex I)’ (2005), Biochem.
Soc. Trans. 33,836-839 Part 4.
Bardischewsky, F.,
Quentmeier, A., Rother, D.,Hellwig, P., Kostka, S., Friedrich C.G., ‘Sulfur
dehydrogenase of Paracoccuspantotrophus: the molybdoproteindiheme
cytochrome c-complex isactive without the heme c2
domain’ (2005) Biochemistry44, 7024-7034.
Richter, O.M., Dürr,
K.L., Kannt, A., Ludwig,B., Scandurra, F.M., Giuffre, A., Sarti, P.,
Hellwig, P., ‘Probing the accessof protons to the K-pathway in the
Paracoccus denitrificans cytochrome coxidase’ (2005) Eur. J.
Biochem, FEBS J. 272, 404-12.
2004:
Zhang, J., Hellwig, P.,
Osborne, J.P., Gennis,R.B., ‘Arginine-391 in subunit I of the cytochrome bd
quinol oxidasefrom E. coli stabilizes the reduced form of heme b558
and isessential for quinol oxidase activity’ (2004) J. Biol. Chem. 279,
53980-7.
Quentmeier, A.,
Hellwig, P., Bardischewsky, F.,Friedrich C.G., ‘Sulfide dehydrogenase
activity of the monomeric flavoproteinSoxF of Paracoccus pantotrophus’(2004)
Biochemistry 43,14696-703.
Ritter, M., Palsdottir,
H., Abe, M., Mäntele, W.,Hunte, C., Miyoshi, H., Hellwig P., ‘Direct
evidence for the interaction ofstigmetellin with an acidic group in the bc1
complex from S.cerevisiae as monitored by FTIR difference
spectroscopy and specific 13Clabeling’ (2004) Biochemistry
43,8439-8446.
Wolpert, M., Maneg, O.,
Ludwig, B., Hellwig,P., ‘Electrochemical and FT-IR spectroscopic
characterization of the CuAcenter in the cytochrome c
oxidase from T. thermophilus for thespectral range from 1800-500 cm-1’
(2004) Biopolymers 74, 73-76.
Hellwig, P., Stolpe,
S., Friedrich,T.,‘FTIR spectroscopic study on the conformational
reorganisation in E. colicomplex I due to redox-driven proton
translocation’ (2004) Biopolymers 74, 69-72.
2003:
Quentmeier, A.,
Hellwig, P., Bardischewsky, F.,Grelle, G., Kraft, R., Friedrich C.G.,
‘Sulfur oxidation in Paracoccuspantotrophus: Interaction of the
sulfur-binding protein Sox YZ with thedimanganese SoxB protein’ (2003) Biochem.
Biophys. Res. Commun. 312, 1011-1018.
Ritter, M., Anderka,
O., Ludwig, B., Mäntele,W., Hellwig, P., ‘Electrochemical and FTIR
spectroscopic characterization ofthe bc1 complex from P.
Denitrificans’ (2003)Biochemistry 42, 12391-12399.
Hellwig, P. Gomes, C.,
Texeira, M., ‘FTIRspectroscopic characterization of the cytochrome aa3
from Acidianusambivalens: evidence for the involvement of acidic
residues in redoxcoupled proton translocation.’ (2003) Biochemistry 42,
6179-6184.
Flemming, D., Hellwig,
P., Friedrich T.,‘Involvement of tyrosines 114 and 139 of subunit NuoB in
the proton pathwayaround cluster N2 in E. coli NADH:ubiquinone
oxidoreductase’ (2003) Journalof Biological Chemistry 278,3055-3062.
2002:
Hellwig, P., Soulimane,
T., Mäntele, W.,‘Electrochemical, FTIR and UV/Vis spectroscopic properties
of the caa3oxidase from T. Thermophilus’(2002) European
Journal of Biochemistry 269, 4830-4838.
Hellwig, P., Yano, T.,
Ohnishi, T., Gennis,R.B., ‘Identification of the residues involved in
stabilization of thesemiquinone radical in the high affinity UQ binding
site in cytochrome bo3from E. coli by
site-directed mutagenesis and EPR spectroscopy’ (2002) Biochemistry41,
10675-10679.
Hellwig, P., Pfitzner,
U., Behr, J., Rost, B.,Pessavento, R. P., von Donk, W., Gennis, R.B.,
Michel, H., Ludwig, B., Mäntele,W., ‘Vibrational modes of tyrosines in
cytochrome c oxidase from P.denitrificans: FTIR and
electrochemical studies on Tyr-D4-labeled and onTyr280His and Tyr35Phe
mutant enzymes’ (2002) Biochemistry 41, 9116-25.
Zhang, J., Gennis,
R.B., Oettmeier, W., Hellwig,P., ‘FTIR spectroscopic evidence for the
involvement of an acidic residue inquinone binding in cytochrome bd
from E. coli’ (2002) Biochemistry41, 4612-4617.
Barquera, B., Hellwig,
P., Zhou, W.,Morgan, J.E., Häse, C.C., Gosink, K.K., Nilges, M.,
Bruesehoff, P., Roth, A.,Lancaster, C.R.D., Gennis, R.B., ‘Purification and
Characterization of theRecombinant Na+ - Translocating NADH:
Quinone Oxidoreductase From Vibriocholerae’ (2002) Biochemistry
41, 3781-9.
2001:
Zhang, J., Hellwig, P.,
Osborne, J.P., Huang,H.W., Moenne-Loccoz, P., Konstantinov, A.A., Gennis,
R.B., ‘Site-directedmutagenesis at the highly conserved region near the
Q-loop of cytochrome bdquinol oxidase in Escherichia coli
specifically perturbs heme b595’(2001) Biochemistry
40, 8548-8556.
Hellwig, P., Rost, B.,
Mäntele, W., ‘Redoxdependent conformational changes in the mixed valence
form of the cytochrome coxidase from P. denitrificans: The
reorganization of glutamic acid 278is coupled to the electron transfer
from/to heme a and the binuclearcenter’ (2001) Spectrochimica
Acta Part A: Mol. & Biomol. 57/5, 1123-1131.
Hellwig, P., Barquera,
B., Gennis, R.B.,‘Direct Evidence for the Protonation of Aspartate-75,
Proposed to be at aQuinol Binding Site, upon Reduction of Cytochrome bo3
fromE. coli’ (2001) Biochemistry 40,1077-82.
2000:
Friedrich, T., Brors,
B., Hellwig, P.,Kintscher, L., Rasmussen, T., Scheide, D., Schulte, U.,
Mäntele, W., Weiss, H.,‘Characterization of two novel redox groups in the
respiratory NADH:ubiquinoneoxidoreductase (complex I)’ (2000) Biochim.
Biophys. Acta. 1459, 305-309.
Behr J., Michel H.,
Mäntele W., Hellwig P.,‘Functional properties of the heme propionates in
cytochrome c oxidasefrom Paracoccus denitrificans. Evidence
from FTIR difference spectroscopyand site-directed mutagenesis’ (2000) Biochemistry
39, 1356-63.
Hellwig, P., Scheide,
D., Bungert, S., Mäntele,W., Friedrich, T., ‘FT-IR spectroscopic
characterization of NADH:ubiquinoneoxidoreductase (complex I) from Escherichia
coli: oxidation of FeScluster N2 is coupled with the protonation of an
aspartate or glutamate sidechain’ (2000) Biochemistry 39,
10884-91.
1999:
Kuglstatter, A.,
Hellwig, P., Fritzsch, G.,Wachtveitl, J., Oesterhelt, D., Mäntele, W., Michel,
H., ‘Identification of ahydrogen bond in the Phe M197 -> Tyr mutant
reaction center of thephotosynthetic purple bacterium Rhodobacter
sphaeroides by X-raycrystallography and FTIR spectroscopy.’ (1999) FEBS
Lett. 463,169-74.
Hellwig, P., Mogi, T.,
Tomson, F.L., Gennis,R.B., Iwata, J., Miyoshi, H., Mäntele, W.,
‘Vibrational modes of ubiquinone in cytochrome bo3 from Escherischia
coli identified byFourier Transform difference spectroscopy and
specific 13Clabeling’(1999) Biochemistry 38,
14683-14689.
Hellwig, P., Soulimane,
T., Buse, G., Mäntele,W., ‘Similarities and dissimilarities in the
structure-function relationbetween the cytochrome c oxidase from
bovine heart and from Paracoccusdenitrificans as revealed by FT-IR
spectroscopy’ (1999) FEBS Lett. 458, 83-86.
Kannt, A., Pfitzner,
U., Ruitenberg, M.,Hellwig, P., Ludwig, B., Mäntele, W., Fendler K.,
Michel, H., ‘Mutation ofArg-54 strongly influences heme composition and
rate and directionality ofelectron transfer in Paracoccus denitrificans
cytochrome coxidase’ (1999) J. Biol. Chem. 274,
37974-81.
Hellwig, P., Soulimane,
T., Buse, G., Mäntele,W., ‘Electrochemical, FT-IR and UV/VIS spectroscopic
properties of the ba3oxidase from T. Thermophilus’
(1999) Biochemistry 38, 9648-9658.
Rost, B., Behr, J.,
Richter, O.-M.H., Hellwig,P., Ludwig, B., Michel, H., Mäntele, W.,
‘Time-resolved FT-IR studies on the COadduct of Paracoccus denitrificans
cytochrome c oxidase:comparison of the fully reduced and the mixed
valence form’ (1999) Biochemistry38, 7565-71.
Hellwig, P., Grzybek
S., Behr, J., Ludwig, B.,Michel, H., Mäntele, W., ‘Electrochemical and
UV/VIS/IR-Spectroscopic Analysisof Heme a and a3
Redox Reactions in the Cytochrome cOxidase from Paracoccus
denitrificans: Separation of Heme a and a3
contributions and assignments of vibrational modes’ (1999) Biochemistry38,
1685-1694.
1998:
Hellwig, P.,
Ostermeier, C., Ludwig, B.,Michel, H., Mäntele, W., ‘Electrochemically
induced FT-IR difference spectra ofthe two- and four-subunit cytochrome c
oxidase from P. denitrificansreveal identical conformational changes
upon redox transitions’ (1998) Biochim.Biophys. Acta. 1409,
107-112.
Behr, J., Hellwig, P.,
Mäntele, W., Michel, H.,‘Redox dependent changes at the heme propionates in
the cytochrome coxidase from Paracoccus denitrificans: direct
evidence from FTIRdifference spectra and heme propionate 13C
labelling’ (1998) Biochemistry37, 7400-7406.
Hellwig, P., Behr, J.,
Ostermeier, C., Richter,O.-M.H., Pfitzner, U., Odenwald, A., Ludwig, B.,
Michel, H., Mäntele, W.,‘Involvement of glutamic acid 278 in the redox
reaction of the cytochrome coxidase from Paracoccus denitrificans
investigated by FT-IRspectroscopy’ (1998) Biochemistry 37,7390-7399.
updated
02 Sept 2009
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